Expression level of Wnt signaling components possibly influences the biological behavior of colorectal cancer in different age groups

Heinrich B K Seidler; Masanori Utsuyama; Sakae Nagaoka; Tamiko Takemura; Masanobu Kitagawa; Katsuiku Hirokawa

doi:10.1016/j.yexmp.2003.12.004

Expression level of Wnt signaling components possibly influences the biological behavior of colorectal cancer in different age groups

Exp Mol Pathol. 2004 Jun;76(3):224-33. doi: 10.1016/j.yexmp.2003.12.004.

Authors

Heinrich B K Seidler¹, Masanori Utsuyama, Sakae Nagaoka, Tamiko Takemura, Masanobu Kitagawa, Katsuiku Hirokawa

Affiliation

¹ Department of Pathology and Immunology, Aging and Developmental Sciences, Graduate School of Medicine, Tokyo Medical and Dental University, Bunkyo, Tokyo 113-8519, Japan.

PMID: 15126105
DOI: 10.1016/j.yexmp.2003.12.004

Abstract

Advancing of age apparently influences the behavior of colorectal cancer (CRC). The pattern of activation and expression of Wnt target genes may influence the behavior of the cancer. In the present study, the level of activation of some elements of Wnt signaling was evaluated and correlated with the patient's age and clinicopathological characteristics of the tumor. Beta-catenin and c-Myc mRNA expressions were evaluated by semiquantitative real-time PCR, and subcellular localization of the beta-catenin protein was evaluated by immunohistochemistry. Patients aged 70-84 tended to have locally advanced disease more frequently than younger patients. The same group of patients also more frequently had high nuclear expression of beta-catenin protein and higher expression of c-Myc mRNA. Beta-catenin mRNA had a rather constant expression with advancing of age. High nuclear expression of beta-catenin and high expression of c-Myc were apparently also correlated with locally advanced disease. We concluded that the level of Wnt signaling activation might influence the behavior of the disease in different age groups.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / genetics
Adenocarcinoma / metabolism
Adenocarcinoma / pathology
Adenocarcinoma, Mucinous / genetics
Adenocarcinoma, Mucinous / metabolism
Adenocarcinoma, Mucinous / pathology
Adult
Age Distribution
Aged
Aged, 80 and over
Aging
Cell Nucleus / metabolism
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / metabolism
Colorectal Neoplasms / pathology
Cytoskeletal Proteins / genetics*
Cytoskeletal Proteins / metabolism
Female
Gene Expression Regulation, Neoplastic*
Humans
Immunoenzyme Techniques
Male
Middle Aged
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / pathology
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins c-myc / genetics*
Proto-Oncogene Proteins c-myc / metabolism
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Trans-Activators / genetics*
Trans-Activators / metabolism
Wnt Proteins
Zebrafish Proteins*
beta Catenin

Substances

CTNNB1 protein, human
Cytoskeletal Proteins
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-myc
RNA, Messenger
Trans-Activators
Wnt Proteins
Zebrafish Proteins
beta Catenin