Abstract
Humans with mutations in either DCX or LIS1 display nearly identical neuronal migration defects, known as lissencephaly. To define subcellular mechanisms, we have combined in vitro neuronal migration assays with retroviral transduction. Overexpression of wild-type Dcx or Lis1, but not patient-related mutant versions, increased migration rates. Dcx overexpression rescued the migration defect in Lis1+/- neurons. Lis1 localized predominantly to the centrosome, and after disruption of microtubules, redistributed to the perinuclear region. Dcx outlined microtubules extending from the perinuclear "cage" to the centrosome. Lis1+/- neurons displayed increased and more variable separation between the nucleus and the preceding centrosome during migration. Dynein inhibition resulted in similar defects in both nucleus-centrosome (N-C) coupling and neuronal migration. These N-C coupling defects were rescued by Dcx overexpression, and Dcx was found to complex with dynein. These data indicate Lis1 and Dcx function with dynein to mediate N-C coupling during migration, and suggest defects in this coupling may contribute to migration defects in lissencephaly.
Copyright the Rockefeller University Press
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Alkyl-2-acetylglycerophosphocholine Esterase
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Animals
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Brain / abnormalities
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Brain / cytology
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Brain / metabolism
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Cell Differentiation / genetics
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Cell Movement / genetics
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Cell Nucleus / metabolism*
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Cell Nucleus / ultrastructure
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Cells, Cultured
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Centrosome / metabolism*
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Centrosome / ultrastructure
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Doublecortin Domain Proteins
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Doublecortin Protein
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Dyneins / genetics
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Dyneins / metabolism*
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Gene Expression Regulation, Developmental / genetics
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Macromolecular Substances
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Mice
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Microtubule-Associated Proteins / genetics
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Microtubule-Associated Proteins / metabolism*
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Microtubules / genetics
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Microtubules / metabolism
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Microtubules / ultrastructure
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Mutation / genetics
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Nervous System Malformations / genetics
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Nervous System Malformations / metabolism
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Nervous System Malformations / physiopathology
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Neurons / cytology
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Neurons / metabolism*
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Neuropeptides / genetics
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Neuropeptides / metabolism*
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Up-Regulation / genetics
Substances
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DCX protein, human
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Dcx protein, mouse
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Doublecortin Domain Proteins
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Doublecortin Protein
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Macromolecular Substances
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Microtubule-Associated Proteins
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Neuropeptides
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1-Alkyl-2-acetylglycerophosphocholine Esterase
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Pafah1b1 protein, mouse
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Dyneins