DNA methylation of multiple genes and clinicopathological relationship of non-small cell lung cancers

Tetsuro Hanabata; Kazunori Tsukuda; Shinichi Toyooka; Masaaki Yano; Motoi Aoe; Itaru Nagahiro; Yoshifumi Sano; Hiroshi Date; Nobuyoshi Shimizu

DNA methylation of multiple genes and clinicopathological relationship of non-small cell lung cancers

Oncol Rep. 2004 Jul;12(1):177-80.

Authors

Tetsuro Hanabata¹, Kazunori Tsukuda, Shinichi Toyooka, Masaaki Yano, Motoi Aoe, Itaru Nagahiro, Yoshifumi Sano, Hiroshi Date, Nobuyoshi Shimizu

Affiliation

¹ Department of Cancer and Thoracic Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.

PMID: 15201980

Abstract

Aberrant methylation of 5' CpG islands is thought to play an important role in the inactivation of tumor suppressor genes in several types of cancers. In non-small cell lung cancer (NSCLC), several genes are known to be frequently methylated and the correlation of their methylation with clinical features has been studied. We determined the methylation of p16, CDH13 and RAR-beta which were reported to be methylated frequently in NSCLCs and HPP-1 which was known to be methylated in other types of cancers. The correlation between methylation and clinicopathological features were examined. The frequencies of methylation in NSCLCs were 20% for p16, 37% for CDH13, 34% for RAR-beta, and 13% for HPP1. The methylation of p16 is correlated with smoking history and methylation of HPP1 was significantly more frequent in adenocarcinomas than in squamous cell carcinomas. This is the first description of aberrant methylation of the HPP1 gene in lung cancers and our data support the previous reports on methylation in NSCLCs and association with smoking.

MeSH terms

Adenocarcinoma / classification
Adenocarcinoma / genetics
Aged
Base Sequence
Biomarkers, Tumor
Cadherins / genetics
Carcinoma, Non-Small-Cell Lung / classification*
Carcinoma, Non-Small-Cell Lung / genetics*
Carcinoma, Non-Small-Cell Lung / pathology
Carcinoma, Non-Small-Cell Lung / surgery
Carcinoma, Squamous Cell / classification
Carcinoma, Squamous Cell / genetics
Cyclin-Dependent Kinase Inhibitor p16 / genetics
DNA Methylation*
DNA Primers
DNA, Neoplasm / genetics*
Dinucleoside Phosphates / genetics
Female
Genes, Tumor Suppressor
Humans
Lung Neoplasms / classification*
Lung Neoplasms / genetics*
Lung Neoplasms / pathology
Lung Neoplasms / surgery
Male
Membrane Proteins / genetics
Middle Aged
Neoplasm Proteins / genetics
Receptors, Retinoic Acid / genetics

Substances

Biomarkers, Tumor
Cadherins
Cyclin-Dependent Kinase Inhibitor p16
DNA Primers
DNA, Neoplasm
Dinucleoside Phosphates
H-cadherin
Membrane Proteins
Neoplasm Proteins
Receptors, Retinoic Acid
TMEFF2 protein, human
retinoic acid receptor beta
cytidylyl-3'-5'-guanosine