A human cellular protein activity (OF-1), which binds herpes simplex virus type 1 origin, contains the Ku70/Ku80 heterodimer

J Virol. 2004 Jul;78(14):7839-42. doi: 10.1128/JVI.78.14.7839-7842.2004.

Abstract

In an effort to identify host proteins involved in herpes simplex virus type 1 replication, monkey and human cellular protein activities (called OF-1) that bind the viral replication origin, oriS, have been described. We show by mass spectrometry that the DNA-binding component of human OF-1 contains Ku70 and Ku80 proteins.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Nuclear / chemistry*
  • Antigens, Nuclear / metabolism
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / metabolism*
  • Dimerization
  • Herpesvirus 1, Human / physiology
  • Humans
  • Ku Autoantigen
  • Molecular Sequence Data
  • Replication Origin / genetics
  • Replication Origin / physiology*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Viral Proteins / metabolism
  • Virus Replication

Substances

  • Antigens, Nuclear
  • DNA-Binding Proteins
  • OF-1 protein
  • Viral Proteins
  • Xrcc6 protein, human
  • Ku Autoantigen