Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma

World J Gastroenterol. 2004 Jul 1;10(13):1975-8. doi: 10.3748/wjg.v10.i13.1975.

Abstract

Aim: To analyze loss of heterozygosity (LOH) and homozygous deletion on p53 gene (exon2-3, 4 and 11), chromosome 10q22-10q23 and 22q11.2 -22q12.1 in human hepatocellular carcinoma (HCC).

Methods: PCR and PCR-based microsatellite polymorphism analysis techniques were used.

Results: LOH was observed at D10S579 (10q22-10q23) in 4 of 20 tumors (20%), at D22S421 (22q11.2-22q12.1) in 3 of 20(15%), at TP53.A (p53 gene exon 2-3) in 4 of 20(20%), at TP53.B (p53 gene exon 4) in 6 of 20(30%), and at TP53.G (p53 gene exon 11) in 0 of 20(0%). Homozygous deletion was detected at 10q22-10q23(8/20; 40%), 22q11.2-22q12.1(8/20; 40%), p53 gene exon 2-3(0/20;0%), p53 gene exon 4(6/20; 30%), and p53 gene exon 11(2/20; 10%).

Conclusion: There might be unidentified tumor suppressor genes on chromosome 10q22-10q23 and 22q11.2-22q12.1 that contribute to the pathogenesis and development of HCC.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Hepatocellular / genetics*
  • Chromosomes, Human, Pair 10*
  • Chromosomes, Human, Pair 17
  • Chromosomes, Human, Pair 22*
  • Exons
  • Gene Deletion
  • Homozygote
  • Humans
  • Liver Neoplasms / genetics*
  • Loss of Heterozygosity*
  • Microsatellite Repeats
  • Tumor Suppressor Protein p53 / genetics*

Substances

  • Tumor Suppressor Protein p53