ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells

FEBS Lett. 2004 Jul 16;570(1-3):7-12. doi: 10.1016/j.febslet.2004.06.010.

Abstract

The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this study, we investigated the cellular behaviors of HepG2 cells with exogenous ING4. Interestingly, the overexpression of ING4 negatively regulated the cell growth with significant G2/M arrest of cell cycle, and moreover, enhanced the cell apoptosis triggered by serum starvation in HepG2 cells. Furthermore, the exogenous ING4 could upregulate endogenous p21 and Bax in HepG2 cells, not in p53-deficient Saos-2 cells, suggesting that G2/M arrest induced by ING4 could be mediated by the increased p21 expression in a p53-dependent manner, although there is no significant increase of p53 expression in HepG2 cells. Moreover, HepG2 cells with exogenous ING4 could significantly increase cell death, as exposed to some DNA-damage agents, such as etoposide and doxorubicin, implying that ING4 could enhance chemosensitivity to certain DNA-damage agents in HepG2 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyltransferases / metabolism
  • Animals
  • Apoptosis
  • Cell Cycle
  • Cell Cycle Proteins / metabolism
  • Cell Death
  • Cell Division
  • Cell Line
  • DNA / metabolism
  • DNA Damage*
  • Dose-Response Relationship, Drug
  • Female
  • Flow Cytometry
  • G2 Phase
  • Histone Acetyltransferases
  • Homeodomain Proteins
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mitosis
  • NF-kappa B / metabolism
  • Open Reading Frames
  • Plasmids / metabolism
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2*
  • Proto-Oncogene Proteins p21(ras) / metabolism
  • Time Factors
  • Transcription Factor RelA
  • Transcription Factors
  • Tumor Suppressor Protein p53 / metabolism
  • Tumor Suppressor Proteins / metabolism
  • Tumor Suppressor Proteins / physiology*
  • bcl-2-Associated X Protein
  • p300-CBP Transcription Factors

Substances

  • BAX protein, human
  • Bax protein, mouse
  • Cell Cycle Proteins
  • Homeodomain Proteins
  • ING4 protein, human
  • NF-kappa B
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Transcription Factor RelA
  • Transcription Factors
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Proteins
  • bcl-2-Associated X Protein
  • DNA
  • Acetyltransferases
  • Histone Acetyltransferases
  • p300-CBP Transcription Factors
  • p300-CBP-associated factor
  • HRAS protein, human
  • Proto-Oncogene Proteins p21(ras)