Abstract
A methylation-specific polymerase chain reaction technique was used to investigate aberrant promoter methylation of RASSF1A and HIC-1 genes in circulating extracellular DNA (exDNA) from the blood of breast cancer and fibroadenoma patients. Methylated DNA could be detected in the exDNA eluted from the surface of erythrocytes and leukocytes, even in the samples where no methylated DNA could be detected in plasma. The data obtained demonstrate that cell surface bound exDNA provides a valuable source of material for early noninvasive cancer diagnostics and monitoring.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Breast Neoplasms / blood*
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Breast Neoplasms / diagnosis
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Breast Neoplasms / genetics
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Breast Neoplasms / metabolism*
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DNA Methylation
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DNA, Neoplasm / blood*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism*
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Erythrocytes / metabolism
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Feasibility Studies
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Female
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Fibroadenoma / blood*
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Fibroadenoma / diagnosis
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Fibroadenoma / genetics
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Fibroadenoma / metabolism*
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Humans
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Kruppel-Like Transcription Factors
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Leukocytes / metabolism
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Polymerase Chain Reaction
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Promoter Regions, Genetic
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Transcription Factors / genetics
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Tumor Suppressor Proteins / genetics
Substances
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DNA, Neoplasm
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Hic1 protein, mouse
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Kruppel-Like Transcription Factors
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RASSF1 protein, human
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Transcription Factors
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Tumor Suppressor Proteins