Molecular genetic polymorphism study was undertaken in two endogamous ethnic groups of Maharashtra in Western India at two microsatellites i.e., TPO, a tetranucleotide repeat locus and CD4, a pentanucleotide repeat locus. The two ethnic groups studied were Konkanastha Brahmins and Marathas belonging to Indo-European language family. Eighty-two random, unrelated individuals were genotyped for the locus TPO, whereas for CD4 locus, 79 individuals were genotyped.