Reduced tissue immigration of monocytes by neuropeptide Y during endotoxemia is associated with Y2 receptor activation

Heike Nave; Sammy Bedoui; Frank Moenter; Jan Steffens; Melanie Felies; Thomas Gebhardt; Rainer H Straub; Reinhard Pabst; Mira Dimitrijevic; Stanislava Stanojevic; Stephan von Hörsten

doi:10.1016/j.jneuroim.2004.05.009

Reduced tissue immigration of monocytes by neuropeptide Y during endotoxemia is associated with Y2 receptor activation

J Neuroimmunol. 2004 Oct;155(1-2):1-12. doi: 10.1016/j.jneuroim.2004.05.009.

Authors

Heike Nave¹, Sammy Bedoui, Frank Moenter, Jan Steffens, Melanie Felies, Thomas Gebhardt, Rainer H Straub, Reinhard Pabst, Mira Dimitrijevic, Stanislava Stanojevic, Stephan von Hörsten

Affiliation

¹ Department of Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Str.1, 30625 Hannover, Germany. nave.heike@mh-hannover.de

PMID: 15342191
DOI: 10.1016/j.jneuroim.2004.05.009

Abstract

Neuropeptide Y (NPY) increases survival in experimental septic shock, which might be mediated by cardiovascular and/or immunological effects. To study the latter hypothesis, we monitored blood leukocyte subsets over 96 h after intravenous (i.v.) application of LPS in chronically i.v.-cannulated rats. LPS induced a dramatic leukopenia at 4 h after challenge, which was blunted in NPY-treated animals by stabilizing granulocyte and T-lymphocyte numbers. In addition, NPY treatment prevented tissue immigration of monocytes at early time points and consecutively mobilized activated monocytes from the third day after challenge. RT-PCR and in vitro adhesion studies provided evidence for a NPY Y2 receptor-mediated effect on monocytes. Thus, NPY treatment has profound receptor-specific effects on the migration and adhesion of leukocytes under endotoxemic conditions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis / drug effects
Apoptosis / immunology
Cell Adhesion / drug effects
Cell Adhesion / genetics
Cells, Cultured
Chemotaxis, Leukocyte / drug effects*
Chemotaxis, Leukocyte / immunology
Down-Regulation / drug effects
Down-Regulation / immunology
Endotoxemia / chemically induced
Endotoxemia / drug therapy*
Endotoxemia / physiopathology
Flow Cytometry
Free Radicals / metabolism
Injections, Intravenous
Leukopenia / chemically induced
Leukopenia / physiopathology
Lipopolysaccharides
Male
Monocytes / drug effects*
Monocytes / pathology
Monocytes / physiology
Neuropeptide Y / metabolism
Neuropeptide Y / pharmacology*
RNA, Messenger / drug effects
RNA, Messenger / metabolism
Rats
Rats, Inbred Lew
Receptors, Neuropeptide Y / genetics
Receptors, Neuropeptide Y / metabolism*
T-Lymphocytes / drug effects
T-Lymphocytes / immunology
T-Lymphocytes / pathology
Tumor Necrosis Factor-alpha / drug effects
Tumor Necrosis Factor-alpha / metabolism

Substances

Free Radicals
Lipopolysaccharides
Neuropeptide Y
RNA, Messenger
Receptors, Neuropeptide Y
Tumor Necrosis Factor-alpha
neuropeptide Y2 receptor