Objective: To study the expression of Bcl-2 (B-cell lymphoma/leukemia-2) mRNA and Bcl-2 protein in nasal mucusa of rat in allergic rhinitis (AR) model.
Method: Twenty male or female SD rats, weighing 180-220 g, free of nasal diseases, were randomly divided into two groups. AR model was established through intraperitoneal injection of ovabumin (OVA) as well as nasal challenge with OVA. The nasal mucusa thus obtained from two groups were studied by HE, in situ hybridization histochemical staining of Bcl-2 mRNA and immunohistochemical staining of bcl-2/bax protein.
Result: The positive expression of Bcl-2 mRNA and Bcl-2/bax protein was presented in the glandular cells,epithelium cells and eosinophils. The staining density of Bcl-2 mRNA and Bcl-2 protein in AR rats increased significantly as compared with those in normal controls. No significant differences of Bax protein expression in two groups were found.
Conclusion: Bcl-2 mRNA and Bcl-2 protein were up-regulated in the mucusa of AR rats.