Objective: To study the C242T mutation frequency of the cytochrome b4 (P22phox) in Chinese and to further ascertain the relationship between the polymorphism of P22Phphox C242T, vitamin E and coronary heart disease (CHD).
Methods: 151 patients with CHD and 254 controls were genotyped for the P22phox C242T polymorphism by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, the concentrations of the vitamin E in plasma were measured by reversed-phase high performance liquid chromatography (RP-HPLC).
Results: The study shows that the allele frequency of C and T is 92.72% and 7.28% in the whole people and the polymorphism of P22phox C242T is associated with CHD in Chinese. Vitamin E is protective against CHD and there is effect on the progress of CHD in the interaction of the polymorphism of P22phox C242T and vitamin E. By stepwise Logistic regression analysis the results suggest that male, smoking, non-drinking, hypertension, diabetes, stroke of parents and low concentration of vitamin E in plasma are the possible risk factors in CHD.
Conclusion: The polymorphism of P22phox C242T is associated with CHD in Chinese. Vitamin E is protective against CHD and there is effect on the progress of CHD in the interaction of the polymorphism of P22phox C242T and vitamin E.