Abstract
Interleukin-11, a cytokine with multiple biological activities, has been shown to stimulate the proliferation and to support the long-term growth of human myeloma cell lines. Despite this, no expression of the interleukin-11alpha receptor has so far been demonstrated in myeloma cells. We have investigated the expression of interleukin-11alpha receptor and interleukin-11 at the level of mRNA and protein product in bone marrow mononuclear cells isolated from patients with multiple myeloma using reverse-transcriptase polymerase chain reaction and flow cytometry. The mRNA for interleukin-11alpha receptor and/or the corresponding protein were identified in 9 of 15 patients with multiple myeloma. In contrast, the interleukin-11 was not detected in any of the patients examined.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADP-ribosyl Cyclase / biosynthesis*
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ADP-ribosyl Cyclase 1
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Adult
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Aged
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Aged, 80 and over
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Antigens, CD / biosynthesis*
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Bone Marrow Cells / metabolism*
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Cell Proliferation
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Female
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Flow Cytometry
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Humans
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Interleukin-11 Receptor alpha Subunit
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Leukocytes, Mononuclear / metabolism*
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Male
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Membrane Glycoproteins
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Middle Aged
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Multiple Myeloma / metabolism*
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Plasma Cells / metabolism
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RNA, Messenger / metabolism
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Receptors, Interleukin / biosynthesis*
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Receptors, Interleukin-11
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Reverse Transcriptase Polymerase Chain Reaction
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Time Factors
Substances
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Antigens, CD
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IL11RA protein, human
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Interleukin-11 Receptor alpha Subunit
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Membrane Glycoproteins
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-11
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ADP-ribosyl Cyclase
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CD38 protein, human
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ADP-ribosyl Cyclase 1