Methylation analysis of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma

Leuk Res. 2005 Jan;29(1):47-51. doi: 10.1016/j.leukres.2004.05.004.

Abstract

We investigated methylation status of the adenomatous polyposis coli (APC) gene in adult T-cell leukemia/lymphoma (ATL). APC methylation was found in 15 of 31 (48%) primary samples, and 2 of 4 (50%) ATL cell lines. Methylation of the APC gene occurred more frequently in acute ATL (12/21) (57%) than chronic ATL (1/8) (13%) (P = 0.03). APC was not expressed in the APC-methylated ATL cell line ST1. Demethylation with 5-azacytidine treatment restored APC expression in the ST1 cell line. Our data show that hypermethylation of the APC gene is involved in the pathogenesis of ATL.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenomatous Polyposis Coli Protein / genetics*
  • Azacitidine / pharmacology
  • Cell Line, Tumor
  • CpG Islands / genetics
  • DNA Methylation*
  • Disease Progression
  • Humans
  • Leukemia-Lymphoma, Adult T-Cell / genetics*
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Adenomatous Polyposis Coli Protein
  • Azacitidine