Abstract
The results presented demonstrate the expression of pfkfb4 gene in adult testis and in a mouse spermatogonia germ cell line (GC-1spg). The genomic organization of the human pfkfb4 gene shows the existence of 14 exons and 13 introns, spanning 45 kb. A detailed analysis of the 5'-flanking region by transient transfection assays with different 5'-deletion promoter constructs in GC-1spg and mouse sertoli cells (TM-4), allows us to define the minimal promoter unit, containing several GC-rich and ETF sequences along the first -141 nucleotides involved in basal expression. This gene is activated by serum and chemical hypoxia (CoCl(2) treatment) whereas beta-estradiol decreases its expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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5' Flanking Region / genetics*
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Animals
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Base Sequence
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Cell Line
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Exons / genetics
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Gene Expression
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Gene Expression Regulation / drug effects
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Genes, Reporter
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Germ Cells / metabolism
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Humans
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Introns / genetics
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Luciferases / genetics
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Male
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Mice
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Molecular Sequence Data
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Phosphofructokinase-2 / biosynthesis
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Phosphofructokinase-2 / genetics*
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Promoter Regions, Genetic / genetics*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Rats
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Sequence Deletion / genetics
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Spermatogonia / cytology
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Spermatogonia / metabolism*
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Testis / chemistry
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Testis / metabolism
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Tissue Distribution
Substances
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RNA, Messenger
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Luciferases
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Phosphofructokinase-2