Abstract
Candida albicans PALS-green fluorescent protein (GFP) reporter strains were inoculated into mice in a disseminated candidiasis model, and GFP production was monitored by immunohistochemistry and reverse transcription-PCR (RT-PCR). GFP production from the ALS1 and ALS3 promoters was detected immunohistochemically. ALS1, ALS2, ALS3, ALS4, and ALS9 transcription was detected by RT-PCR, further identifying ALS genes expressed in this model.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Agglutinins / genetics*
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Agglutinins / metabolism
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Animals
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Candida albicans / genetics
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Candida albicans / metabolism
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Candida albicans / pathogenicity*
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Candidiasis / microbiology*
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Disease Models, Animal
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Female
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Fungal Proteins / genetics*
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Fungal Proteins / metabolism
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Fungemia / microbiology*
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Gene Expression*
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Genes, Fungal
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Immunohistochemistry
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Mice
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Mice, Inbred BALB C
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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ALS1 protein, Candida albicans
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ALS3 protein, Candida albicans
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Agglutinins
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Fungal Proteins
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Green Fluorescent Proteins