Abstract
The Mullerian ducts are strongly influenced by natural estrogen, estradiol (E2) and diethylstilbestrol (DES) in their development. We screened E2 and DES responsive genes using a microarray analysis in human primitive Mullerian duct cell line, EMTOKA cells expressed estrogen receptor (ER) beta. c-myc oncogene and other target genes expression was detected in cells treated by high-dose DES, but ER antagonist ICI 182,780 could not prevent c-myc induction above. Results of our present study suggested the presence of ER independent pathway in oncogenes induction process by high-dose DES treatment in a human primitive Mullerian duct cell line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Hormonal / pharmacology*
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Cell Culture Techniques
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Diethylstilbestrol / pharmacology*
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Dose-Response Relationship, Drug
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Estradiol / pharmacology
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Female
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Gene Expression Profiling*
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Genes, myc
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Humans
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Mullerian Ducts / drug effects*
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Mullerian Ducts / physiology
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Oligonucleotide Array Sequence Analysis
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Receptors, Estrogen / biosynthesis*
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Transcription, Genetic
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Uterine Neoplasms / chemically induced
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Uterine Neoplasms / genetics
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Vaginal Neoplasms / chemically induced
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Vaginal Neoplasms / genetics
Substances
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Antineoplastic Agents, Hormonal
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Receptors, Estrogen
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Estradiol
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Diethylstilbestrol