Isolation and primary structure of a novel chromogranin A-derived peptide, WE-14, from a human midgut carcinoid tumour

W J Curry; C Shaw; C F Johnston; L Thim; K D Buchanan

doi:10.1016/0014-5793(92)80266-j

Isolation and primary structure of a novel chromogranin A-derived peptide, WE-14, from a human midgut carcinoid tumour

FEBS Lett. 1992 Apr 27;301(3):319-21. doi: 10.1016/0014-5793(92)80266-j.

Authors

W J Curry¹, C Shaw, C F Johnston, L Thim, K D Buchanan

Affiliation

¹ Department of Medicine, Queen's University of Belfast, N. Ireland, UK.

PMID: 1577173
DOI: 10.1016/0014-5793(92)80266-j

Abstract

The primary structure of a novel human chromogranin A-derived tetradecapeptide, WE-14, possessing N-terminal tryptophanyl (W) and C-terminal glutamyl (E) residues was isolated from a hepatic metastasis of an human ileal carcinoid tumour. Human and bovine WE-14 are structurally identical, while rat, mouse and porcine analogues exhibit 93% homology. WE-14 is flanked by paired basic residues (KR) in all known chromogranin A sequences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Carcinoid Tumor / metabolism*
Carcinoid Tumor / pathology
Chromatography, Gel
Chromatography, High Pressure Liquid
Chromogranin A
Chromogranins / metabolism*
Humans
Intestinal Neoplasms / metabolism*
Intestinal Neoplasms / pathology
Liver Neoplasms / metabolism
Liver Neoplasms / secondary
Male
Middle Aged
Molecular Sequence Data
Neoplasm Proteins / genetics*
Neoplasm Proteins / isolation & purification
Protein Processing, Post-Translational
Radioimmunoassay

Substances

CHGA protein, human
Chromogranin A
Chromogranins
Neoplasm Proteins
chromogranin A, mouse
chromogranin A, rat
chromogranin A-derived peptide, WE-14