Abstract
The expression status of the three cyclin D genes (CCND1, CCND2 and CCND3), the two cyclin D-dependent kinase genes (CDK4 and CDK6) and the p16(INK4a) gene was studied in a series of 47 Wilms' tumors, 16 normal mature kidneys and two fetal kidneys. We showed predominant overexpression of CCND2 and CDK4 compared to CCND1/D3 and CDK6 respectively. We found a specific correlation between relapse and CDK4 overexpression, but not CDK6 overexpression. We did not identify any methylation of the p16(INK4a) promoter. This suggests that dysregulation of CCND2 and CDK4 plays a specific role in WT tumorigenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cyclin D1 / genetics
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Cyclin D2
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase 6
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Cyclin-Dependent Kinase Inhibitor p16 / genetics
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Cyclin-Dependent Kinases / genetics*
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Cyclins / genetics*
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DNA Methylation
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Humans
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Kidney / embryology
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Kidney / enzymology
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Kidney Neoplasms / genetics*
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Promoter Regions, Genetic
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Proto-Oncogene Proteins / genetics*
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Wilms Tumor / genetics*
Substances
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CCND2 protein, human
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Cyclin D2
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Cyclin-Dependent Kinase Inhibitor p16
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Cyclins
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Proto-Oncogene Proteins
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Cyclin D1
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CDK4 protein, human
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CDK6 protein, human
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinase 6
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Cyclin-Dependent Kinases