Abstract
Bone marrow endothelial cells (EC) from patients with multiple myeloma (MM) were found to express and secrete higher amounts of the CXC-chemokines CXCL8/interleukin (IL)-8, CXCL11/interferon-inducible T-cell alpha chemoattractant (I-TAC), CXCL12/stromal cell-derived factor (SDF)-1alpha, and CCL2/monocyte chemotactic protein(MCP)-1 than EC from human umbilical vein (HUVEC), considered as a healthy counterpart. Paired plasma cells and several MM cell lines expressed cognate receptors of each chemokine to a variable extent. When cells were exposed to chemokines, CXCL8/IL-8 and CXCL12/SDF-1alpha stimulated their proliferation and all chemokines stimulated cell chemotaxis. It is suggested that angiogenesis also favours MM progression through the release of CXC-chemokines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Blotting, Western / methods
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Bone Marrow Cells / immunology*
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Bone Marrow Cells / metabolism
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Case-Control Studies
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Cell Communication
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cells, Cultured
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Chemokine CCL2 / genetics
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Chemokine CCL2 / metabolism
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Chemokine CCL2 / pharmacology
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Chemokine CXCL11
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Chemokine CXCL12
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Chemokines, CXC / genetics
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Chemokines, CXC / metabolism*
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Chemokines, CXC / pharmacology
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Chemotaxis, Leukocyte / drug effects
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Endothelial Cells / immunology*
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Endothelial Cells / metabolism
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Female
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Flow Cytometry
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Humans
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Interleukin-8 / genetics
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Interleukin-8 / metabolism
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Interleukin-8 / pharmacology
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Male
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Middle Aged
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Multiple Myeloma / immunology*
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Multiple Myeloma / physiopathology
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Plasma Cells / immunology*
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Receptors, Chemokine / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Stimulation, Chemical
Substances
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CCL2 protein, human
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CXCL11 protein, human
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CXCL12 protein, human
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Chemokine CCL2
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Chemokine CXCL11
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Chemokine CXCL12
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Chemokines, CXC
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Interleukin-8
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Receptors, Chemokine