Heterogeneous expression pattern of pro- and anti-apoptotic factors in myeloid progenitor cells of patients with severe congenital neutropenia treated with granulocyte colony-stimulating factor

Br J Haematol. 2005 Apr;129(2):275-8. doi: 10.1111/j.1365-2141.2005.05428.x.

Abstract

Apoptosis is accelerated in the myeloid progenitor cells of patients with severe congenital neutropenia (CN). Granulocyte colony-stimulating factor (G-CSF) increases neutrophil numbers in most CN patients. The effect of G-CSF on apoptosis in CN was analysed by apoptosis rate and expression of anti- and pro-apoptotic factors. G-CSF-treated patients showed higher apoptosis frequency, lower expression of bcl-2 and bcl-xL, but higher expression of bfl-1/A1 and mcl-1. Caspase 9 was highly expressed in patients and controls after G-CSF administration. Thus, G-CSF acts on apoptosis regulation, but additional mechanisms leading to the increase of neutrophil numbers must be assumed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antigens, CD / immunology
  • Antigens, Differentiation, Myelomonocytic / immunology
  • Apoptosis / drug effects
  • Biomarkers / analysis
  • Case-Control Studies
  • Caspase 9
  • Caspases / analysis
  • Caspases / genetics
  • Child, Preschool
  • Female
  • Fluorescent Antibody Technique
  • Gene Expression / drug effects
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Humans
  • Infant
  • Male
  • Minor Histocompatibility Antigens
  • Myeloid Cell Leukemia Sequence 1 Protein
  • Myeloid Progenitor Cells / drug effects
  • Myeloid Progenitor Cells / immunology
  • Myeloid Progenitor Cells / metabolism*
  • Neoplasm Proteins / analysis
  • Neoplasm Proteins / genetics
  • Neutropenia / congenital*
  • Proto-Oncogene Proteins c-bcl-2 / analysis
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sialic Acid Binding Ig-like Lectin 3
  • Stimulation, Chemical
  • bcl-X Protein

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • BCL2-related protein A1
  • BCL2L1 protein, human
  • Biomarkers
  • CD33 protein, human
  • Minor Histocompatibility Antigens
  • Myeloid Cell Leukemia Sequence 1 Protein
  • Neoplasm Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Sialic Acid Binding Ig-like Lectin 3
  • bcl-X Protein
  • Granulocyte Colony-Stimulating Factor
  • CASP9 protein, human
  • Caspase 9
  • Caspases