Up-regulation of interleukin (IL)-6 receptor gene expression in vitro and in vivo in IL-6 deprived myeloma cells

FEBS Lett. 1992 May 4;302(1):35-8. doi: 10.1016/0014-5793(92)80278-o.

Abstract

Myeloma cells absolutely require interleukin-6 (IL-6) for growing in vivo in patients with multiple myeloma and exogenous IL-6-dependent myeloma cell lines have been reproducibly obtained. In this study we show a dramatic up-regulation of the IL-6 receptor (gp80 chain) gene expression in myeloma cell lines following the removal of exogenous IL-6. Such a regulation was also known to occur in IL-6-deprived myeloma cells in vivo in three patients who were treated with optimal doses of anti-IL-6 monoclonal antibodies. The direct effect of IL-6 on IL-6 receptor gene expression in myeloma cells was further confirmed by adding IL-6 to an autonomously growing myeloma cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Interleukin-6 / deficiency
  • Interleukin-6 / metabolism*
  • Multiple Myeloma / genetics*
  • Multiple Myeloma / metabolism
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-6
  • Tumor Cells, Cultured
  • Up-Regulation*

Substances

  • Interleukin-6
  • Receptors, Immunologic
  • Receptors, Interleukin-6