Acute promyelocytic leukemia with PML-RARA fusion on i(17q) and therapy-related acute myeloid leukemia

Cancer Genet Cytogenet. 2005 Jun;159(2):129-36. doi: 10.1016/j.cancergencyto.2004.09.019.

Abstract

We describe a patient with acute promyelocytic leukemia (APL) and the karyotype 46,XX,i(17)(q10) with PML-RARA fusion gene detected by fluorescence in situ hybridization (FISH) and nested reverse transcriptase-polymerase chain reaction (RT-PCR). FISH using dual-color translocation probes for PML (promyelocytic leukemia) and RARA (retinoic acid receptor-alpha) showed fusion signal for PML-RARA on both arms of i(17q). The patient attained complete remission (CR) with all-trans retinoic acid treatment and became PML-RARA negative. One year later, while PML-RARA negative on FISH and RT-PCR, the patient presented with thrombocytopenia. Bone marrow examination suggested an acute monoblastic leukemia (AML-M5a) including the karyotype 46,XX,t(8;16) (p11.2;p13.3),inv(11)(p15q22 approximately q23)[11]/47,idem,+i(8)(q10)[9]. She is currently in CR. The occurrence of therapy related acute leukemia after successful therapy for APL is an emerging problem.

Publication types

  • Case Reports

MeSH terms

  • Antineoplastic Agents / adverse effects*
  • Chromosomes, Human, Pair 17*
  • Female
  • Humans
  • In Situ Hybridization, Fluorescence
  • Isochromosomes*
  • Karyotyping
  • Leukemia, Monocytic, Acute / chemically induced*
  • Leukemia, Promyelocytic, Acute / genetics*
  • Middle Aged
  • Neoplasm Proteins / genetics*
  • Nuclear Proteins / genetics
  • Oncogene Proteins, Fusion / genetics*
  • Promyelocytic Leukemia Protein
  • Receptors, Retinoic Acid / genetics*
  • Retinoic Acid Receptor alpha
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors / genetics
  • Translocation, Genetic
  • Tretinoin / adverse effects*
  • Tumor Suppressor Proteins

Substances

  • Antineoplastic Agents
  • Neoplasm Proteins
  • Nuclear Proteins
  • Oncogene Proteins, Fusion
  • Promyelocytic Leukemia Protein
  • RARA protein, human
  • Receptors, Retinoic Acid
  • Retinoic Acid Receptor alpha
  • Transcription Factors
  • Tumor Suppressor Proteins
  • promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein
  • PML protein, human
  • Tretinoin