Abstract
The authors examined the usefulness of nested PCR (N-PCR) to detect Mycobacterium tuberculosis (MTB) DNA in CSF for assessing the clinical course of tuberculous meningitis (TBM). N-PCR successfully detected MTB DNA in all nine CSF samples from patients with suspected TBM. During anti-tuberculosis treatments, N-PCR results converted from positive to negative, correlating with the improvement of the patient's clinical condition.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Antitubercular Agents / therapeutic use
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Cerebrospinal Fluid / chemistry*
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Cerebrospinal Fluid / microbiology
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DNA, Bacterial / analysis*
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DNA, Bacterial / genetics
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Disease Progression
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Female
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Humans
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Male
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Mycobacterium tuberculosis / genetics*
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / trends
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Predictive Value of Tests
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Treatment Outcome
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Tuberculosis, Meningeal / cerebrospinal fluid*
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Tuberculosis, Meningeal / diagnosis*
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Tuberculosis, Meningeal / microbiology
Substances
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Antitubercular Agents
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DNA, Bacterial