Abstract
Gene expression in frontal, occipital, and hippocampal regions of rat brains at 15 min of ischemic injury was studied in a rat model by producing focal cerebral ischemia through middle cerebral artery (MCA) occlusion without reperfusion. Catalase, epithelial glycoprotein (EGP-314), cytochrome C oxidase-subunit 1, ribosomal L31 protein, and ceruloplasmin were found to be differentially expressed. Specific primers were designed to study this newly reported brain EGP-314, a cellular adhesion molecule involved in cell-cell and cell-extracellular matrix interactions and related with cytoskeletal organization, differentiation, and proliferation. In the frontal and occipital lobes, EGP-314 expression was low in control and ischemic conditions and increased in sham injured conditions, whereas in the hippocampal region its expression was induced only by ischemia. In situ hybridization and immunohistochemistry revealed that EGP-314 mRNA and the protein were present in the ischemic hippocampus pyramidal neurons. DNA fragmentation was demonstrated by TUNEL and LM-PCR analysis in hippocampus region. TUNEL positive pyramidal neurons were observed at 15 min of ischemia. DNA ladder was found at 12 and 15 min of ischemia.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Neoplasm / genetics*
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Antigens, Neoplasm / metabolism*
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Apoptosis / genetics
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Brain / metabolism*
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Brain / physiopathology
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Brain Ischemia / genetics*
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Brain Ischemia / metabolism*
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Brain Ischemia / physiopathology
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Catalase / genetics
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Catalase / metabolism
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Cerebral Infarction / genetics
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Cerebral Infarction / metabolism
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Cerebral Infarction / physiopathology
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Ceruloplasmin / genetics
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Ceruloplasmin / metabolism
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Disease Models, Animal
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Electron Transport Complex IV / genetics
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Electron Transport Complex IV / metabolism
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Gene Expression Regulation / genetics*
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Hippocampus / metabolism
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Hippocampus / pathology
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Infarction, Middle Cerebral Artery / genetics
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Infarction, Middle Cerebral Artery / metabolism
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Infarction, Middle Cerebral Artery / physiopathology
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Male
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Nerve Degeneration / genetics
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Nerve Degeneration / metabolism
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Nerve Degeneration / physiopathology
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Pyramidal Cells / metabolism
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Pyramidal Cells / pathology
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Rats
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Rats, Wistar
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Ribosomal Proteins / genetics
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Ribosomal Proteins / metabolism
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Time Factors
Substances
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Antigens, Neoplasm
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EGP314 antigen
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Ribosomal Proteins
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ribosomal protein L31
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Catalase
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Ceruloplasmin
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Electron Transport Complex IV