Abstract
Hydroquinone-alpha-glucoside was synthesised from hydroquinone and maltose as glucosyl donor by transglucosylation in a water system with alpha-glucosidase from baker's yeast. Only one phenolic -OH group was alpha-anomer-selectively glucosylated. The optimum conditions for transglucosylation reaction were at 30 degrees C for 20 h with 50 mM: hydroquinone and 1.5 M: maltose in 100 mM: sodium citrate/phosphate buffer at pH 5.5. The glucoside was obtained at 0.6 mg/ml with a 4.6% molar yield with respect to hydroquinone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Glucosides / analysis
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Glucosides / biosynthesis*
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Glycosylation
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Hydrogen-Ion Concentration
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Hydroquinones / metabolism
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Magnetic Resonance Spectroscopy / methods
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Maltose / metabolism
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae Proteins / isolation & purification
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Saccharomyces cerevisiae Proteins / metabolism
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Temperature
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Time Factors
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alpha-Glucosidases / isolation & purification
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alpha-Glucosidases / metabolism*
Substances
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Glucosides
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Hydroquinones
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Saccharomyces cerevisiae Proteins
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hydroquinoneglucoside
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Maltose
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alpha-Glucosidases
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hydroquinone