Abstract
To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic sequence (ITS). We detected two different hsp65 genes and correspondingly two ITS sequevars belonging to M. abscessus type I and type II.
MeSH terms
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Adolescent
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Adult
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Bacterial Proteins / genetics*
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Chaperonin 60
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Chaperonins / genetics*
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Child
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Cystic Fibrosis / microbiology*
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DNA, Ribosomal Spacer / analysis*
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Female
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Genetic Variation*
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Humans
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Male
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Molecular Sequence Data
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Mycobacterium Infections, Nontuberculous / microbiology*
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Mycobacterium chelonae / classification*
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Mycobacterium chelonae / genetics
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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RNA, Ribosomal, 16S / genetics
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RNA, Ribosomal, 23S / genetics
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Sequence Analysis, DNA
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Sputum / microbiology
Substances
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Bacterial Proteins
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Chaperonin 60
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DNA, Ribosomal Spacer
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RNA, Ribosomal, 16S
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RNA, Ribosomal, 23S
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heat-shock protein 65, Mycobacterium
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Chaperonins
Associated data
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GENBANK/AY603553
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GENBANK/AY603554
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GENBANK/AY635142
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GENBANK/AY635143