Intra- and interpatient variability of the hsp65 and 16S-23S intergenic gene region in Mycobacterium abscessus strains from patients with cystic fibrosis

J Clin Microbiol. 2005 Jul;43(7):3500-3. doi: 10.1128/JCM.43.7.3500-3503.2005.

Abstract

To establish the exact pathogenic role of Mycobacterium abscessus in cystic fibrosis (CF), molecular tests are required for accurate identification. Forty-eight M. abscessus isolates from seven patients with CF were analyzed by sequence analysis for sequence variants within the hsp65 gene and the 16S-23S intergenic sequence (ITS). We detected two different hsp65 genes and correspondingly two ITS sequevars belonging to M. abscessus type I and type II.

MeSH terms

  • Adolescent
  • Adult
  • Bacterial Proteins / genetics*
  • Chaperonin 60
  • Chaperonins / genetics*
  • Child
  • Cystic Fibrosis / microbiology*
  • DNA, Ribosomal Spacer / analysis*
  • Female
  • Genetic Variation*
  • Humans
  • Male
  • Molecular Sequence Data
  • Mycobacterium Infections, Nontuberculous / microbiology*
  • Mycobacterium chelonae / classification*
  • Mycobacterium chelonae / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S / genetics
  • RNA, Ribosomal, 23S / genetics
  • Sequence Analysis, DNA
  • Sputum / microbiology

Substances

  • Bacterial Proteins
  • Chaperonin 60
  • DNA, Ribosomal Spacer
  • RNA, Ribosomal, 16S
  • RNA, Ribosomal, 23S
  • heat-shock protein 65, Mycobacterium
  • Chaperonins

Associated data

  • GENBANK/AY603553
  • GENBANK/AY603554
  • GENBANK/AY635142
  • GENBANK/AY635143