Abstract
Single palpable nodules of the thyroid gland are common in clinical practice; the majority of such lesions are benign. However, noninvasive thyroid nodules that exhibit borderline morphological signs of papillary cancer represent a diagnostic challenge. Rearrangements of the RET oncogene have been proposed as a marker for papillary thyroid cancer. In this chapter, methods for the analysis of the RET oncogene in laser microdissected papillary thyroid cancer tissue are described.
MeSH terms
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Biomarkers, Tumor / biosynthesis
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Biomarkers, Tumor / genetics*
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Carcinoma, Papillary / genetics
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Carcinoma, Papillary / metabolism
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Carcinoma, Papillary / pathology
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Gene Expression Regulation, Neoplastic*
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Humans
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Lasers*
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Microdissection / methods*
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Proto-Oncogene Proteins c-ret / biosynthesis
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Proto-Oncogene Proteins c-ret / genetics*
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Reverse Transcriptase Polymerase Chain Reaction / methods*
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Thyroid Neoplasms / genetics*
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Thyroid Neoplasms / metabolism
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Thyroid Neoplasms / pathology
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Thyroid Nodule / genetics*
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Thyroid Nodule / metabolism
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Thyroid Nodule / pathology
Substances
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Biomarkers, Tumor
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Proto-Oncogene Proteins c-ret
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RET protein, human