Purpose: To study whether interleukin-1 (IL-1) genotypes and/or alleles were associated with aggressive periodontitis (AgP).
Methods: Anti-coagulated peripheral blood samples were obtained from 122 AgP patients and 95 healthy controls. Genomic DNA was extracted from each sample. Single nucleotide polymorphisms (SNPs) at IL-1A +4845 and IL-1B +3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. The polymorphism of a variable number tandem repeat (VNTR) in intron 2 of IL-1RN was detected by PCR amplification and fragment size analysis. The genotypes and/or alleles distribution differences between two groups were analyzed by logistic regression analysis.
Results: The frequencies of A2+ genotype and allele 2 at IL-1A +4845 were significantly increased in male AgP patients compared with male controls (genotype: 18.0% vs 4.3%, adjusted OR=5.58, 95% CI=1.09-28.68, P=0.039; allele: 9.0% vs 2.2%, adjusted OR=4.97, 95% CI=1.01-24.50, P=0.049).
Conclusions: The IL-1A +4845 polymorphism may be associated with AgP susceptibility in Chinese males.