Common polymorphisms in cytokine genes, widely distributed in many populations, may affect gene transcription, leading to individual variations in cytokine levels. Some of those polymorphisms were associated with the risk of graft rejection or autoimmune and parasitic diseases development as well as treatment outcome. In the current study the frequencies of promoter polymorphisms in genes encoding interleukin-2 (-330 G/C), interleukin-4 (-590 C/T), interleukin-6 (-174 G/C), interleukin-10 (-1082 G/A, -592 C/A) and TNFalpha (-307 G/A) have been studied in a sample of 205 unrelated healthy Polish subjects of Caucasian origin. The frequencies of the determined cytokine alleles were as follows: interleukin-2 (-330 G/C): T -64.9%, G -35.1%; interleukin-4 (-590 C/T): C -79.8%, T -20.2%; interleukin-6 (-174 G/C): G -53.4%, C -46.6%; interleukin-10 (-1082 G/A, -819 G/T, -592 C/A): GCC -44.1%, ACC -32.0, ATA -23.9%; TNFalpha (-307 G/A): G -85.1%, A -14.9%. We also compared our results with other populations studied to date, describing some significant differences.