Abstract
Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressure stimulates inflammatory cytokine production and signaling of osteoclastogenic events. Positive pressure enhanced the expression of IL-1alpha mRNA and protein in odontogenic keratocyst epithelial cells, and increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and the epithelial cells. The pressure-induced secretions were inhibited by an interleukin-1 receptor antagonist. Recombinant human interleukin-1alpha (rhIL-1alpha) increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in the fibroblasts. Furthermore, in the fibroblasts, rhIL-1alpha enhanced the expression of macrophage colony-stimulating factor (M-CSF) mRNA, and rhIL-1alpha-induced PGE2 increased the expression of nuclear factor kappaB ligand (RANKL) mRNA. Thus, positive pressure may play a crucial role in odontogenic keratocyst growth via stimulating the expression of IL-1alpha in epithelial cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cells, Cultured
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Dinoprostone / metabolism
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Epithelial Cells / cytology
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Epithelial Cells / metabolism*
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Fibroblasts / cytology
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Fibroblasts / metabolism
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Humans
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Interleukin-1 / metabolism*
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Jaw Diseases / immunology
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Jaw Diseases / metabolism*
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Jaw Diseases / pathology
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Macrophage Colony-Stimulating Factor / genetics
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Macrophage Colony-Stimulating Factor / metabolism
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Matrix Metalloproteinase 1 / metabolism
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Matrix Metalloproteinase 2 / metabolism
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Matrix Metalloproteinase 3 / metabolism*
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Matrix Metalloproteinases / metabolism*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Odontogenic Cysts / immunology
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Odontogenic Cysts / metabolism*
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Odontogenic Cysts / pathology
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Pressure
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RANK Ligand
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RNA, Messenger / analysis
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Receptor Activator of Nuclear Factor-kappa B
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Stress, Mechanical
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Up-Regulation
Substances
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Carrier Proteins
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Interleukin-1
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Membrane Glycoproteins
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RANK Ligand
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RNA, Messenger
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Receptor Activator of Nuclear Factor-kappa B
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TNFRSF11A protein, human
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TNFSF11 protein, human
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Macrophage Colony-Stimulating Factor
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Matrix Metalloproteinases
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Matrix Metalloproteinase 3
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 1
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Dinoprostone