Abstract
Fas and p75 neurotrophin receptors (p75(NTR)) are death receptors that alone induce apoptosis of SH-SY5Y neuroblastoma cell line respectively by Fas ligand or brain-derived neurotrophic factor (BDNF, a p75(NTR) ligand). We report on the modulation of Fas-mediated apoptosis by concomitant p75(NTR) activation. The exposure to both ligands suppressed the apoptotic effect. A co-localisation of Fas and p75(NTR) receptors was evidenced by co-capping and immunoprecipitation assays. Moreover, a caspase-8 inhibitor suppressed the protective effect of the concomitant BDNF and Fas ligand stimulation, suggesting that p75(NTR) and Fas receptors could share common signalling pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis* / drug effects
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Brain-Derived Neurotrophic Factor / pharmacology
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Caspase Inhibitors
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Cell Line, Tumor
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Enzyme Inhibitors / pharmacology
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Fas Ligand Protein / pharmacology
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Models, Biological
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Neuroblastoma / enzymology
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Neuroblastoma / genetics
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Neuroblastoma / metabolism*
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Neuroblastoma / pathology*
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Protein Transport / drug effects
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor, Nerve Growth Factor / genetics
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Receptor, Nerve Growth Factor / metabolism*
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Receptor, trkB / metabolism
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Sphingomyelin Phosphodiesterase / antagonists & inhibitors
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fas Receptor / genetics
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fas Receptor / metabolism*
Substances
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Brain-Derived Neurotrophic Factor
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Caspase Inhibitors
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Enzyme Inhibitors
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FAS protein, human
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Fas Ligand Protein
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RNA, Messenger
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Receptor, Nerve Growth Factor
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fas Receptor
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Receptor, trkB
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Sphingomyelin Phosphodiesterase
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neutral sphingomyelinase-1, human