The zinc-finger protein ZFR is critical for Staufen 2 isoform specific nucleocytoplasmic shuttling in neurons

J Neurochem. 2006 Jan;96(1):105-17. doi: 10.1111/j.1471-4159.2005.03523.x. Epub 2005 Nov 8.

Abstract

In mammalian neurons, transport and translation of mRNA to individual potentiated synapses is believed to occur via a heterogeneous population of RNA granules. To identify components of Staufen2-containing granules, we used the yeast two-hybrid system. A mouse fetal cDNA library was screened with the N-terminal fragment of Staufen2 as bait. ZFR, a three zinc finger protein, was identified as an interacting protein. Confocal microscopy showed that ZFR, although mainly nuclear, was also found in the somatodendritic compartment of primary hippocampal neurons where it localized as granule-like structures. Co-localization with Staufen2 was observed in several granules. Biochemical analyses (immunoprecipitation, cell fractionation) further confirmed the ZFR/Staufen2 association. ZFR was shown to interact with at least the Staufen2(62) isoform, but not with Staufen1. ZFR also co-fractionated with ribosomes and Staufen2(59) and Staufen2(52) in a sucrose gradient. Interestingly, knockdown expression of ZFR through RNA interference in neurons relocated specifically the Staufen2(62), but not the Staufen2(59), isoform to the nucleus. Our results demonstrate that ZFR is a native component of Staufen2-containing granules and likely plays its role during early steps of RNA transport and localization. They also suggest that one of these roles may be linked to Staufen2(62)-containing RNA granule formation in the nucleus and/or to their nucleo-cytoplasmic shuttling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Nucleus / physiology*
  • Cell Nucleus / ultrastructure*
  • Cells, Cultured
  • Cytoplasm / physiology*
  • Cytoplasm / ultrastructure*
  • Cytoplasmic Granules / metabolism
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / genetics
  • Down-Regulation / physiology
  • Fluorescent Antibody Technique
  • Image Processing, Computer-Assisted
  • Immunoprecipitation
  • Lentivirus / genetics
  • Lentivirus / ultrastructure
  • Mice
  • Nerve Tissue Proteins / physiology*
  • Neurons / physiology*
  • Neurons / ultrastructure*
  • RNA Interference
  • RNA-Binding Proteins / metabolism*
  • RNA-Binding Proteins / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection
  • Ultracentrifugation

Substances

  • DNA, Complementary
  • Nerve Tissue Proteins
  • RNA-Binding Proteins
  • ZFR protein, mouse
  • stau2 protein, mouse