Abstract
The Epstein-Barr virus immediate-early protein BZLF1 (Z) has been shown to alter the cellular localization of the promyelocytic leukemia (PML) protein. PML has important implications for growth control, apoptosis, anti-viral effects and many more processes. Here we further examined the relationship between PML and the Epstein-Barr virus Z protein. We examined the effect of Z expression on PML protein levels, and the effect of increased PML protein levels on Z-mediated dispersion of PML bodies. We found that increased levels of PML protein, such as through interferon treatment, were able to suppress Z-mediated PML body dispersion. We also studied the consequences of PML dispersion by Z, by examining p21 transactivation, A20 transactivation, and MHC Class I presentation levels in Z-expressing cells. We found that, while Z-mediated dispersion of PML did not affect MHC Class I presentation, it did alter p21 and A20 expression. In addition, we found that increased levels of PML were able to prevent Z protein binding to mitotic chromosomes. Our work implies that the balance of PML and Z levels in cells may affect how each protein functions.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Artificial Gene Fusion
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Blotting, Western
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Chromosomes / chemistry
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DNA-Binding Proteins / metabolism*
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Gene Expression
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Genes, Reporter
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HeLa Cells
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Histocompatibility Antigens Class I / physiology
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Humans
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Intracellular Signaling Peptides and Proteins
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Luciferases / analysis
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Luciferases / genetics
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Microscopy, Confocal
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Microscopy, Fluorescence
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Neoplasm Proteins / metabolism*
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Nuclear Proteins / metabolism*
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Promyelocytic Leukemia Protein
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Protein Binding
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Proteins / analysis
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Trans-Activators / metabolism*
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Transcription Factors / metabolism*
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Transcriptional Activation
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Tumor Necrosis Factor alpha-Induced Protein 3
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Tumor Suppressor Proteins / analysis
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Tumor Suppressor Proteins / metabolism*
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Viral Proteins / metabolism*
Substances
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BZLF1 protein, Herpesvirus 4, Human
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DNA-Binding Proteins
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Histocompatibility Antigens Class I
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Intracellular Signaling Peptides and Proteins
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Neoplasm Proteins
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Nuclear Proteins
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Promyelocytic Leukemia Protein
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Proteins
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Trans-Activators
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Transcription Factors
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Tumor Suppressor Proteins
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Viral Proteins
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PML protein, human
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Luciferases
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TNFAIP3 protein, human
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Tumor Necrosis Factor alpha-Induced Protein 3