Abstract
Beta amyloid (Abeta) is believed one of the major pathogens of Alzheimer's disease (AD), and the reduction of Abeta is considered a primary therapeutic target. Immunization with Abeta can reduce Abeta burden and pathological features in transgenic AD model mice. Transgenic potato plants were made using genes encoding 5 tandem repeats of Abeta1-42 peptides with an ER retention signal. Amyloid precursor protein transgenic mice (Tg2576) fed with transgenic potato tubers with adjuvant showed a primary immune response and a partial reduction of Abeta burden in the brain. Thus, Abeta tandem repeats can be expressed in transgenic potato plants to form immunologically functional Abeta, and these potatoes has a potential to be used for the prevention and treatment of AD.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alzheimer Disease / genetics*
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Alzheimer Disease / pathology
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Alzheimer Disease / prevention & control*
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Amino Acid Sequence
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Amino Acid Substitution
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Amyloid beta-Peptides / chemistry
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Amyloid beta-Peptides / genetics*
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Amyloid beta-Peptides / immunology
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Amyloid beta-Peptides / isolation & purification
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Amyloid beta-Peptides / metabolism
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Amyloid beta-Peptides / pharmacology
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Amyloid beta-Peptides / therapeutic use*
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Animals
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Brain / pathology
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Cell Line
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Cell Survival / drug effects
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Disease Models, Animal
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Escherichia coli / genetics
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Humans
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Immunization
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Isoleucine / metabolism
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Mice
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Mice, Transgenic
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Models, Biological
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Molecular Sequence Data
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Plants, Genetically Modified
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Plaque, Amyloid / genetics
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Plaque, Amyloid / pathology
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Solanum tuberosum / genetics*
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Solanum tuberosum / metabolism
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Tandem Repeat Sequences / genetics
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Time Factors
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Vaccines / therapeutic use
Substances
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Amyloid beta-Peptides
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Vaccines
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Isoleucine