The prognostic value of cN-II and cN-III enzymes in adult acute myeloid leukemia

Haematologica. 2005 Dec;90(12):1699-701.

Abstract

We analyzed the expression of deoxycytidine kinase (dCK), UMP/CMP-kinase (UMP/CMP-K), nucleotide diphosphokinase (NDPK-B) and 5'-nucleotidases cN-II, cN-III, cdN and mdN by quantitative polymerase chain reaction at diagnosis in leukemic blasts from 96 patients with acute myeloid leukemia (AML) treated with ara-C. Our results show that high mRNA levels of cN-II and low mRNA levels of cN-III are correlated with a worse clinical outcome and suggest that these enzymes may have a role in sensitivity to ara-C in AML patients.

Publication types

  • Letter

MeSH terms

  • 5'-Nucleotidase / biosynthesis
  • 5'-Nucleotidase / genetics*
  • Acute Disease
  • Adult
  • Aged
  • Antimetabolites, Antineoplastic / administration & dosage
  • Antimetabolites, Antineoplastic / pharmacokinetics*
  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Bone Marrow Cells / enzymology
  • Cytarabine / administration & dosage
  • Cytarabine / pharmacokinetics*
  • Drug Resistance, Neoplasm / genetics
  • Female
  • Glycoproteins / biosynthesis
  • Glycoproteins / genetics*
  • Humans
  • Leukemia, Myeloid / drug therapy
  • Leukemia, Myeloid / enzymology*
  • Leukemia, Myeloid / genetics
  • Leukemia, Myeloid / mortality
  • Male
  • Middle Aged
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics*
  • Neoplastic Stem Cells / enzymology*
  • Prognosis
  • Proportional Hazards Models
  • Pyrimidine Nucleotides / metabolism
  • RNA, Messenger / analysis*
  • RNA, Messenger / blood
  • RNA, Neoplasm / analysis*
  • RNA, Neoplasm / blood
  • Reverse Transcriptase Polymerase Chain Reaction
  • Survival Analysis
  • Treatment Outcome

Substances

  • Antimetabolites, Antineoplastic
  • Glycoproteins
  • Neoplasm Proteins
  • Pyrimidine Nucleotides
  • RNA, Messenger
  • RNA, Neoplasm
  • Cytarabine
  • 5'-Nucleotidase
  • NT5C2 protein, human
  • NT5C3A protein, human