Abstract
To clarify the role of GATA transcription factors in ovarian carcinogenesis, we analyzed the expression and methylation states of GATA-4/-5/-6 in eight human ovarian cancer cell lines. GATA-4/-5 were methylated in three and two cell lines without their expression, respectively. Methylation of GATA-4/-5 was also detected in nine and five of 15 primary ovarian cancers, respectively. GATA-6 was not methylated in any cases. We transiently over-expressed GATA-5 in the JHOC-5 cell line using an adenovirus system, resulting in that apoptosis was induced and apoptosis-related genes, such as Apaf-1, were up-regulated. These data suggest that GATA-4/-5 may be involved in ovarian carcinogenesis.
MeSH terms
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Adenocarcinoma, Clear Cell / genetics
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Adenocarcinoma, Clear Cell / metabolism
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Apoptosis
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Carcinoma, Endometrioid / genetics
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Carcinoma, Endometrioid / metabolism
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Cell Proliferation
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Cystadenocarcinoma, Serous / genetics
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Cystadenocarcinoma, Serous / metabolism
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DNA Methylation*
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DNA, Neoplasm / genetics
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DNA, Neoplasm / metabolism
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Female
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GATA4 Transcription Factor / genetics*
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GATA5 Transcription Factor / genetics*
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GATA6 Transcription Factor / genetics*
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Gene Expression Regulation, Neoplastic / physiology*
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Gene Silencing
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Humans
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Ovarian Neoplasms / genetics*
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Ovarian Neoplasms / metabolism
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Promoter Regions, Genetic
Substances
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DNA, Neoplasm
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GATA4 Transcription Factor
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GATA5 Transcription Factor
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GATA6 Transcription Factor