Abstract
Anaplastic large cell lymphoma (ALCL) comprises 10-15% of childhood non-Hodgkin lymphomas (NHL). Systemic ALCL is highly associated with anaplastic lymphoma kinase (ALK) gene translocations with over-expression of ALK protein. We studied ALK rearrangements using fluorescence in situ hybridisation (FISH) and ALK immunohistochemical staining in 43 paediatric systemic ALCLs. FISH (performed on 35 cases) identified a translocation in 29 cases (83%). Immunohistochemistry identified ALK over-expression in 42/43 cases (97%) with the single ALK-negative case demonstrating an ALK rearrangement by FISH, indicating 100% incidence of ALK translocations.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Anaplastic Lymphoma Kinase
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Antigens, CD / analysis
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Chromosomes, Human, Pair 2
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Chromosomes, Human, Pair 5
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Female
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Gene Rearrangement*
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Humans
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Immunohistochemistry / methods
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Immunophenotyping
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In Situ Hybridization, Fluorescence
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Lymphoma, Large B-Cell, Diffuse / genetics*
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Lymphoma, Large B-Cell, Diffuse / pathology
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Male
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Nuclear Proteins / genetics
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Nucleophosmin
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Protein-Tyrosine Kinases / genetics*
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Receptor Protein-Tyrosine Kinases
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Translocation, Genetic
Substances
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Antigens, CD
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Nuclear Proteins
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Nucleophosmin
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ALK protein, human
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Anaplastic Lymphoma Kinase
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases