Abstract
Increasing evidence suggests that lysosomal cysteine proteases cathepsins contribute to the progression of cell apoptosis. Here we found that apoptosis of ovarian cancer cells OV-90 triggered by TNF was cathepsin B-depended. Two cathepsin B binding proteins, bikunin and TSRC1, were identified by yeast two-hybrid method and the interactions were confirmed in vitro and in vivo. Overexpression of bikunin could suppress TNF-induced apoptosis of OV-90 cells, and TSRC1 overexpression had an opposite effect on apoptosis. The presented results suggest that cathepsin B and its interacting proteins, bikunin and TSRC1, are involved in the apoptotic pathway of ovarian cancer cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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ADAMTS Proteins
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Animals
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Apoptosis / drug effects*
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Autocrine Communication / genetics
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Cathepsin B / genetics
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Cathepsin B / metabolism*
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Cell Line, Tumor
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Female
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Gene Expression
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Humans
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism*
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Ovarian Neoplasms / metabolism*
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Thrombospondins / genetics
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Thrombospondins / metabolism*
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Trypsin Inhibitor, Kunitz Soybean / genetics
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Trypsin Inhibitor, Kunitz Soybean / metabolism*
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Tumor Necrosis Factor-alpha / metabolism
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Tumor Necrosis Factor-alpha / pharmacology*
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Two-Hybrid System Techniques
Substances
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ADAMTSL4 protein, human
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Membrane Glycoproteins
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SPINT2 protein, human
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Thrombospondins
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Tumor Necrosis Factor-alpha
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Trypsin Inhibitor, Kunitz Soybean
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Cathepsin B
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ADAMTS Proteins