Abstract
We previously showed that the Spt-Ada-Gcn5-acetyltransferase (SAGA) complex is recruited to the activated HXT2 and HXT4 genes and plays a role in the association of TBP-associated factors. Using the HXT2 and HXT4 genes, we now present evidence for a functional link between Snf1p-dependent activation, recruitment of the SAGA complex, histone H3 removal, and H3 acetylation. Recruitment of the SAGA complex is dependent on the release of Ssn6p-Tup1p repression by Snf1p. In addition, we found that the Gcn5p subunit of the SAGA complex preferentially acetylates histone H3K18 on the HXT2 and HXT4 promoters and that Gcn5p activity is required for removal of histone H3 from the HXT4 promoter TATA region. In contrast, histone H3 removal from the HXT2 promoter does not require Gcn5p. In conclusion, although similar protein complexes are involved, induction of HXT2 and HXT4 displays important mechanistic differences.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Chromatin Assembly and Disassembly*
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DNA-Binding Proteins / physiology
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Glucose Transport Proteins, Facilitative
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Histone Acetyltransferases / physiology
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Histones / metabolism
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Membrane Proteins / genetics*
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Monosaccharide Transport Proteins / genetics*
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Promoter Regions, Genetic*
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Protein Serine-Threonine Kinases / physiology*
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Repressor Proteins / physiology
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / metabolism*
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Saccharomyces cerevisiae Proteins / physiology
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TATA Box
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Trans-Activators / metabolism*
Substances
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DNA-Binding Proteins
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Glucose Transport Proteins, Facilitative
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HXT2 protein, S cerevisiae
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HXT4 protein, S cerevisiae
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Histones
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MIG1 protein, S cerevisiae
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Membrane Proteins
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Monosaccharide Transport Proteins
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Repressor Proteins
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SAGA complex, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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GCN5 protein, S cerevisiae
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Histone Acetyltransferases
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SNF1-related protein kinases
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Protein Serine-Threonine Kinases