The transcripts of SFRP1,CEP63 and EIF4G2 genes are frequently downregulated in transitional cell carcinomas of the bladder

Oncology. 2005;69(6):445-54. doi: 10.1159/000090984. Epub 2006 Jan 12.

Abstract

Objective: The aim of the present study was to identify differentially expressed genes that might be associated with the phenotype of superficial and invasive bladder cancer.

Methods: Differential display reverse transcriptase PCR (DDRT-PCR) was used to compare the expression pattern between normal bladder tissue and 4 groups of transitional cell carcinomas of the bladder regarding clinical stage and grade.

Results: We were able to identify 72 different transcripts, of which 57 (79%) showed homology to known genes, 12 (17%) to hypothetical proteins and 3 (4%) to human expressed sequence tags. Among the differentially expressed genes, SFRP1,CEP63 and EIF4G2 were further validated by quantitative RT-PCR in a series of 50 transitional cell carcinomas. Overall, the transcripts of these three genes were shown to be downregulated in the bladder tumors analyzed. In accordance with the DDRT-PCR results, the SFRP1 transcripts were shown to be downregulated in 90% (45/50) of the bladder tumors as compared with the normal bladder tissue. Although EIF4G2 and CEP63 transcripts exhibited three different expression patterns, downregulation was found in about 50% of the cases analyzed. In addition, downregulation of both CEP63 and EIF4G2 gene transcription was associated with invasive tumors.

Conclusion: The use of DDRT-PCR analysis to compare expression patterns among different subgroups of bladder tumors allowed us to identify a significant number of genes implicated in different cellular pathways that, when up- or downregulated, might play a role in the tumorigenic process of the bladder.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Carcinoma, Transitional Cell / genetics*
  • Cell Cycle Proteins
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • DNA, Neoplasm / analysis
  • Down-Regulation
  • Eukaryotic Initiation Factor-4G / genetics*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics*
  • Male
  • Membrane Proteins / genetics*
  • Middle Aged
  • Neoplasm Proteins / genetics*
  • RNA, Neoplasm / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Transcription, Genetic*
  • Urinary Bladder / metabolism
  • Urinary Bladder Neoplasms / genetics*

Substances

  • CEP63 protein, human
  • Cell Cycle Proteins
  • DNA, Complementary
  • DNA, Neoplasm
  • EIF4G2 protein, human
  • Eukaryotic Initiation Factor-4G
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Neoplasm Proteins
  • RNA, Neoplasm
  • SFRP1 protein, human