Abstract
The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with the yeast gene RAR1, isolated elsewhere by complementation of a rar1 mutation involved in the stability of plasmids with weak ARS. In addition, we show that mevalonate kinase is not a rate-limiting enzyme; however its sensitivity to FFP could be a key regulatory mechanism in the sterol pathway of yeast.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Ergosterol / biosynthesis
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Genes, Fungal
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Molecular Sequence Data
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Phosphotransferases (Alcohol Group Acceptor)*
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Phosphotransferases / antagonists & inhibitors
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Phosphotransferases / genetics*
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Phosphotransferases / metabolism
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Polyisoprenyl Phosphates / pharmacology
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Promoter Regions, Genetic
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Sesquiterpenes
Substances
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Polyisoprenyl Phosphates
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Sesquiterpenes
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geranyl pyrophosphate
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farnesyl pyrophosphate
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Phosphotransferases
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Phosphotransferases (Alcohol Group Acceptor)
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mevalonate kinase
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Ergosterol
Associated data
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GENBANK/S39723
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GENBANK/S39727
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GENBANK/S39777
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GENBANK/S60919
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GENBANK/S60920
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GENBANK/X55875
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GENBANK/X58894
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GENBANK/X62724
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GENBANK/X62725
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GENBANK/X62800