Abstract
The phenotype of HIV-1 gp120 envelope derived from renal epithelium and peripheral blood mononuclear cells (PBMC) of patients with HIV-associated nephropathy was investigated in vitro. Chimeric viruses were derived from kidney or blood and used to infect primary CD4+T cells, cell lines expressing single co-receptors and a renal epithelial cell line HPT-1. HIV-1 variants derived from renal epithelium were dual tropic whereas simultaneously derived viruses from PBMC were R5-tropic. Utilization of alternative co-receptors CCR3, BONZO and BOB, also differed.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
AIDS-Associated Nephropathy / genetics
-
AIDS-Associated Nephropathy / pathology
-
AIDS-Associated Nephropathy / virology*
-
Cell Line
-
Chimerism
-
Epithelial Cells / virology
-
HIV Envelope Protein gp120 / genetics*
-
HIV-1 / genetics
-
HIV-1 / physiology*
-
Humans
-
Kidney Tubules / pathology
-
Kidney Tubules / virology
-
Leukocytes, Mononuclear / virology
-
Phenotype
-
Receptors, CCR3
-
Receptors, CXCR6
-
Receptors, Chemokine / metabolism
-
Receptors, Cytokine / metabolism
-
Receptors, G-Protein-Coupled / metabolism
-
Receptors, Virus / metabolism
-
Tropism / physiology*
-
Virus Replication / genetics*
Substances
-
CCR3 protein, human
-
CXCR6 protein, human
-
HIV Envelope Protein gp120
-
Receptors, CCR3
-
Receptors, CXCR6
-
Receptors, Chemokine
-
Receptors, Cytokine
-
Receptors, G-Protein-Coupled
-
Receptors, Virus