Abstract
Interferon regulatory factor-1 (IRF-1) mRNA expression was examined in specific cell populations (BMMC, CD34+ and CD71+) derived from 45 MDS patients and 20 controls. All the MDS cell populations, presented an identical IRF-1 mRNA expression pattern, characterized by the absence of full-length IRF-1 mRNA and presence of multiple alternative transcripts. The most common deletions involved exons 2 and 3. Two novel truncated IRF-1 protein forms were detected in MDS BMMC. IRF-1-induced iNOS mRNA expression was exclusively detected in BMMC having full-length transcript. The expression of IRF-1 truncated mRNA and protein forms might be a critical event in the development of MDS.
Publication types
-
Clinical Trial
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aged
-
Aged, 80 and over
-
Alternative Splicing / genetics*
-
Antigens, CD
-
Antigens, CD34
-
Cells, Cultured
-
Exons / genetics
-
Female
-
Gene Expression Regulation / genetics*
-
Hematopoietic Stem Cells / metabolism*
-
Hematopoietic Stem Cells / pathology
-
Humans
-
Interferon Regulatory Factor-1 / biosynthesis
-
Interferon Regulatory Factor-1 / genetics*
-
Male
-
Middle Aged
-
Myelodysplastic Syndromes / genetics*
-
Myelodysplastic Syndromes / metabolism
-
Myelodysplastic Syndromes / pathology
-
Nitric Oxide Synthase Type II / biosynthesis
-
Nitric Oxide Synthase Type II / genetics
-
RNA, Messenger / biosynthesis
-
RNA, Messenger / genetics
-
Receptors, Transferrin
Substances
-
Antigens, CD
-
Antigens, CD34
-
CD71 antigen
-
Interferon Regulatory Factor-1
-
RNA, Messenger
-
Receptors, Transferrin
-
Nitric Oxide Synthase Type II