Abstract
The PML protein localizes to regions of the nucleus known as nuclear bodies or PODs. However, in t(15;17) Acute Promyelocytic Leukemia (APL) blasts, PML is found in a micro-punctate pattern. In order to test the hypothesis that delocalization of PML from PODs is necessary for APL, we investigated the interaction of the t(5;17) APL fusion protein NPM-RAR with PML. NPM-RAR localizes diffusely throughout the nucleoplasm. NPM-RAR does not alter the localization of PML in transfected HeLa cells, and does not associate with PML in vitro. These studies suggest that NPM-RAR does not interact with PML.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
COS Cells
-
Chlorocebus aethiops
-
Chromosomes, Human, Pair 17 / genetics*
-
Chromosomes, Human, Pair 5 / genetics*
-
HeLa Cells
-
Humans
-
Leukemia, Promyelocytic, Acute / genetics*
-
Neoplasm Proteins / genetics*
-
Neoplasm Proteins / immunology
-
Neoplasm Proteins / metabolism
-
Nuclear Proteins / genetics*
-
Nuclear Proteins / immunology
-
Nuclear Proteins / metabolism
-
Oncogene Proteins, Fusion / genetics*
-
Oncogene Proteins, Fusion / immunology
-
Polymerase Chain Reaction / methods
-
Promyelocytic Leukemia Protein
-
Protein Binding
-
Sensitivity and Specificity
-
Transcription Factors / genetics*
-
Transcription Factors / immunology
-
Transcription Factors / metabolism
-
Translocation, Genetic / genetics*
-
Tumor Suppressor Proteins / genetics*
-
Tumor Suppressor Proteins / immunology
-
Tumor Suppressor Proteins / metabolism
Substances
-
NPM-RARalpha protein, human
-
Neoplasm Proteins
-
Nuclear Proteins
-
Oncogene Proteins, Fusion
-
Promyelocytic Leukemia Protein
-
Transcription Factors
-
Tumor Suppressor Proteins
-
PML protein, human