Abstract
We investigated whether p53 plays a role in UV induced apoptosis in melanoma. UVA and UVB induced apoptosis in dose dependent and wild type p53 melanoma cells were much more vulnerable than the mutant cells, indicating that p53 played a role in UV-induced apoptosis in melanoma. No difference in p53 expression pattern between the primary and matched metastatic melanomas was noticed in tumour tissue or cell lines from the same patients. Our findings indicate that expression of p53 plays a role in UV-induced apoptosis in melanoma cells, but not important in melanoma progression from primary to metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / radiation effects*
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Cyclin-Dependent Kinase Inhibitor p21 / metabolism
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Disease Progression
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Dose-Response Relationship, Radiation
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Gene Expression Regulation, Neoplastic / radiation effects*
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Humans
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Immunoenzyme Techniques
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Melanoma / metabolism*
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Melanoma / pathology
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Mutation / genetics
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Proto-Oncogene Proteins c-bcl-2 / metabolism
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Skin Neoplasms / metabolism*
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Skin Neoplasms / pathology
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Tumor Cells, Cultured / radiation effects
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Tumor Suppressor Protein p53 / physiology*
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Ultraviolet Rays*
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bcl-2-Associated X Protein / metabolism
Substances
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BAX protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Proto-Oncogene Proteins c-bcl-2
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein