Abstract
Nuclear actin and myosin 1 (NM1) are key regulators of gene transcription. Here, we show by biochemical fractionation of nuclear extracts, protein-protein interaction studies and chromatin immunoprecipitation assays that NM1 is part of a multiprotein complex that contains WICH, a chromatin remodelling complex containing WSTF (Williams syndrome transcription factor) and SNF2h. NM1, WSTF and SNF2h were found to be associated with RNA polymerase I (Pol I) and ribosomal RNA genes (rDNA). RNA interference-mediated knockdown of NM1 and WSTF reduced pre-rRNA synthesis in vivo, and antibodies to WSTF inhibited Pol I transcription on pre-assembled chromatin templates but not on naked DNA. The results indicate that NM1 cooperates with WICH to facilitate transcription on chromatin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / chemistry
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism*
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Cell Nucleus / chemistry
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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Chromatin Assembly and Disassembly / genetics*
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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HeLa Cells
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Humans
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Intracellular Signaling Peptides and Proteins / genetics
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Intracellular Signaling Peptides and Proteins / metabolism*
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Intracellular Signaling Peptides and Proteins / physiology
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Myosin Type I / chemistry
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Myosin Type I / genetics
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Myosin Type I / metabolism*
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Nuclear Proteins / physiology
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Protein Binding / genetics
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RNA Polymerase I / biosynthesis
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RNA Polymerase I / chemistry
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RNA Polymerase I / genetics*
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic*
Substances
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BAZ1B protein, human
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Chromosomal Proteins, Non-Histone
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Intracellular Signaling Peptides and Proteins
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NMI protein, human
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Nuclear Proteins
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Transcription Factors
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RNA Polymerase I
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Adenosine Triphosphatases
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Myosin Type I
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SMARCA5 protein, human
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MYO1C protein, human