Characterization of the t(17;19) translocation by gene-specific fluorescent in situ hybridization-based cytogenetics and detection of the E2A-HLF fusion transcript and protein in patients' cells

Jenny Yeung; Helena Kempski; Michael Neat; Simon Bailey; Owen Smith; Hugh J M Brady

Characterization of the t(17;19) translocation by gene-specific fluorescent in situ hybridization-based cytogenetics and detection of the E2A-HLF fusion transcript and protein in patients' cells

Haematologica. 2006 Mar;91(3):422-4.

Authors

Jenny Yeung, Helena Kempski, Michael Neat, Simon Bailey, Owen Smith, Hugh J M Brady

PMID: 16531271

Abstract

This is the first report to comprehensively characterize the E2A-HLF fusion generated from the t(17;19)(q22;p13) translocation in childhood B-lineage acute lymphoblastic leukemia. E2A gene rearrangement and E2A-HLF transcript and protein expression were determined using conventional cytogenetics, fluorescent in situ hybridization, reverse transcriptase polymerase chain reaction and Western blotting in leukemic cells from three patients.

Publication types

Comparative Study
Letter
Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Child, Preschool
Chromosomes, Human, Pair 17 / genetics*
Chromosomes, Human, Pair 19 / genetics*
Cytogenetic Analysis / methods
DNA-Binding Proteins / genetics*
Female
Gene Fusion / genetics*
Humans
In Situ Hybridization, Fluorescence / methods*
Male
Oncogene Proteins, Fusion / genetics*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism
Transcription Factors / genetics*
Translocation, Genetic / genetics*

Substances

DNA-Binding Proteins
E2a-Hlf fusion protein, human
Oncogene Proteins, Fusion
Transcription Factors