cDNA cloning of the beta-subunit of the human gastric H,K-ATPase

Biochem Biophys Res Commun. 1991 Oct 15;180(1):39-45. doi: 10.1016/s0006-291x(05)81251-3.

Abstract

A full-length cDNA clone encoding the human gastric H,K-ATPase (EC 3.6.1.36)beta-subunit was isolated from a human gastric mucosal lambda gt10 library using oligonucleotide probes which were based on the cDNA sequence from rat and rabbit H,K-ATPase beta-subunits. The insert was 1407 bp in length and encoded a polypeptide of 291 amino acids with a MW = 33,367 Da. It exhibited 84.2%, 85.6% and 81.3% identity to the H,K-ATPase beta-subunits of rabbit, pig and rat, respectively.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / isolation & purification
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA / genetics*
  • Gastric Mucosa / enzymology*
  • H(+)-K(+)-Exchanging ATPase
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Oligonucleotide Probes
  • Rabbits
  • Rats
  • Sequence Homology, Nucleic Acid
  • Swine

Substances

  • Oligonucleotide Probes
  • DNA
  • Adenosine Triphosphatases
  • H(+)-K(+)-Exchanging ATPase

Associated data

  • GENBANK/M75110