Structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) containing an amide of D-galacturonic acid with L-alanine

Carbohydr Res. 2006 Aug 14;341(11):1969-74. doi: 10.1016/j.carres.2006.03.021. Epub 2006 May 15.

Abstract

The structure of the O-polysaccharide of Proteus mirabilis CCUG 10705 (OF) was determined by chemical analyses along with one- and two-dimensional (1)H and (13)C NMR spectroscopy. The polysaccharide was found to contain an amide of D-galacturonic acid with L-alanine and based on the uniqueness of the O-polysaccharide structure and serological data, it was suggested to classify P. mirabilis OF into a new separate Proteus serogroup, O74. A weak cross-reactivity of P. mirabilis OF and P. mirabilis O5 was observed and accounted for by a similarity of their O-repeating units. The following structure of the polysaccharide of P. mirabilis OF was established: [chemical structure: see text]

MeSH terms

  • Acetylation
  • Alanine / chemistry*
  • Amides / chemistry
  • Animals
  • Carbohydrate Sequence
  • Hexuronic Acids / chemistry*
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / isolation & purification
  • Magnetic Resonance Spectroscopy
  • Methanol / chemistry
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • O Antigens / isolation & purification
  • Proteus mirabilis / chemistry*
  • Proteus mirabilis / classification
  • Rabbits
  • Serotyping

Substances

  • Amides
  • Hexuronic Acids
  • Lipopolysaccharides
  • O Antigens
  • galacturonic acid
  • Alanine
  • Methanol