Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

Yoshio Kusakabe; Satoshi Ohno; Nobutada Tanaka; Masashi Nakamura; Masaru Tsunoda; Tomohisa Moriguchi; Norio Asai; Mitsuo Sekine; Takashi Yokogawa; Kazuya Nishikawa; Kazuo T Nakamura

doi:10.2174/092986606775974465

Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

Protein Pept Lett. 2006;13(4):417-9. doi: 10.2174/092986606775974465.

Authors

Yoshio Kusakabe¹, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa, Kazuo T Nakamura

Affiliation

¹ School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.

PMID: 16712521
DOI: 10.2174/092986606775974465

Abstract

Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNA(Tyr). The crystals belong to a tetragonal space group P4(1)2(1)2 with cell dimensions of a = b = 63.85 Angstrom, and c = 330.3 Angstrom. The asymmetric unit contains one molecule each of yTyrRS and tRNA(Tyr) (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Angstrom resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallization
Crystallography, X-Ray
RNA, Transfer, Tyr / chemistry*
Saccharomyces cerevisiae / enzymology
Tyrosine-tRNA Ligase / chemistry*

Substances

RNA, Transfer, Tyr
Tyrosine-tRNA Ligase